Reverse transcriptase-PCR assay for detection of hog cholera virus

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Reverse transcriptase-PCR assay for detection of hog cholera virus.

A reverse transcriptase-PCR strategy was developed for the detection of hog cholera virus. Hog cholera virus template was amplified from tissue culture fluids and from tissues and blood of infected pigs, but not from samples containing other pestiviruses. Restriction endonuclease analysis identified samples as historic or recent isolates.

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Classical Swine Fever(CSF) or Hog Cholera is one of the most feared and devastating disease of pigs. The disease has become main threat to pig industry in counrties with a dense pig population and is known to cause more deaths in pigs as compared to many other infectious agents. The disease is endemic in many parts of India including Assam. The virus belongs to genus Pestivirus and family Flavi...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1994

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.32.10.2600-2602.1994